Effect of Transfer Factor
Advanced Formulas
Containing E-XF Blends on
Natural Killer (NK) Cell
A 4Life Summary of an Independent NK
Cell Study Report by Calvin W.
McCausland, Ph.D. and Emma Oganova
M.D., Ph.D.
Objective: To determine the extent to
which Transfer Factor E-XF blends
and 4Life Transfer Factor Plus
Advanced Formula increases Natural
Killer (NK) cell activity above
Study Design: The blinded
cytotoxicity study was designed by Dr.
E. Oganova and Dr. C. McCausland.
The independent testing of the coded
samples was done under the direction
of Academician Anatoly Vorobiev,
M.D., Russian Academy of Medical
Sciences (RAMS) and the
experimental work was conducted by.
Dr. M.V. Kisielevsky, Dr. E.O.
Khalturina, at the Russian Cancer
Research Center, RAMS.
Methods: Blood was obtained from
RORC donor station. A standard
for 24 or 48 hours. Next 30 thousand
K-562 tumor cells (erythroblastic
human leukemia) were introduced into
the wells. (Thus, the ratio of effector
and target cells was 2:1. Each sample
was tested in triplicate). The prepara
tions containing both the effector (NK
cells) and target cells (K562 cancer
cells) along with control wells were
again incubated for 18-24 hours under
the same incubator conditions. An
MTT (dye) solution was used to
spectro-photometricly determine the
number of viable cells remaining in
each well. The cytotoxic index (CI)
expressed in % is reported for each
Results: Each of the samples
significantly increased NK cell
activity. The Transfer Factor E-XF
Blend (Advanced Formula) resulted in
method of density gradient was used to
isolate mononuclear cells [NK Cells].
The isolated cells, 60 thousand cells
per well, in 100 ml of culture medium,
were introduced into each well
ofCostar 96 well plates. Then, portions
of coded test samples [Transfer Factor
E-XF blends of selected ratios, 4Life
Transfer Factor Plus E-XF blend and
others samples] were introduced into
the wells at predetermined
concentrations (wt/vol.). An
Interleukin-2 (IL-2) standard was used
to compare effectiveness. The
preparations were incubated in a COzincubator
with a 5% C02 atmosphere,
100% humidity and 37° C
greater NK cell activation (283%) than
the Transfer Factor XF (204%). 4Life
Transfer Factor Plus Advanced
Formula increased NK cell activity by
437%. The drug IL-2 increased NK
cell active by 389%. In the study
activation by 4Life Transfer Factor
Advanced Formula, 4Life Transfer
Factor Plus Advanced Formula and IL-
2 resulted in a kill rate of K562 cancer
cells of 69%, 97% and 88%
respectively with the 48 hour
incubation period giving the greatest
NK cell activation.
As previously reported in earlier publications two 4Life products, 4Life
Transfer Factor Classic and 4Life Transfer Factor Plus, have been
shown to significantly improve NK cell activation, 103% and 248%
respectively. When the transfer factors from patented egg technology
are combined with bovine transfer factors there is a synergistic
activation ofNK cell activity. The improved formulas, 4Life Transfer
Factor and 4Life Transfer Factor Plus Advanced Formulas improve NK
cell activation by 283% and 437% respectively.
4Life researchers and scientists in developing the Advanced Formulas
were confident that the colostrum derived transfer factors when
combined with the egg derived transfer factors (E-XF blends) would
enhance the activity and benefit of the products, but we too were taken
by surprise by the magnitude of the increase. Further research needs to
be done to better understand why this synergism occurs. We are
confident these results will translate into increased benefits to
consumers and increased clinical effect in future studies.
This study was conducted under the direction of Dr. Anatoli Vorobiev
by his colleagues of the Russian Academy of Medical Science. Using
established methods ofcytotoxicity testing, NK cells from humans were
combined with cancer cells and divided into groups of transfer factors
activated NK cells and groups ofunactivated NK cells. The objective
was to find the bovine and egg transfer factor blend(s) (E-XF blends)
that express the greatest NK cell activation. The results enable us to
identify the most potent combination that will provide the greatest
health benefits.
In the control series of the experiments for all of the transfer factor
samples tested there was no direct cytotoxic effect on tumor cells at
any of the concentrations tested. When in the absence of the immune
NK cells the transfer factors are combined directly with the cancer cells
there is no cytotoxic effect on the tumor cells.
Results of this study clearly demonstrated that 4Life Transfer Factor
Advanced Formula and 4Life Transfer Factor Plus Advanced Formula
boosts NK cell activity. The independent research scientists who
conducted this study found the results interesting and so exceptional as
to request further information on the identity of 4Life samples so that
results could be published in professional journals. Statements from
researchers included the following:
“The 4Life sample activated NK cell activity more than the Interleukin-
2 (IL-2) drug used as the standard. Here, we now refer to your sample
as the Golden Interleukin,” stated Dr. Kisielevsky, Russian Academy of
Medical Sciences.
We strongly believe that these results are certain to be reflected in
improved human health resulting from significantly improved immune
response. It is our hope that people everywhere will find that 4Life
Transfer Factor Advanced Formula and 4Life Transfer Factor Plus
Advanced Formula will provide safe broad-spectrum immune support
that is unmatched in any other health supplement today.

February 8, 2008 - Posted by | 4LIFE, BARAH | , , , , , , , , , ,

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